EASYCOUNTER YC VITALITY KIT

PN: YC-VT

1 KIT FOR 50 TESTS



Price: 99€

BUY


ballot Features


In many cases, toxic effects of chemical or physical factors do not lead directly to cell death. Such factors may cause a number of morphological, intracellular, or metabolic alterations that will result in the inability of a cell to divide, yet the cell itself may still be alive. The method for assessing cell viability provide information only on alive and dead cells in the whole population, but do not provide information about the number of vitality cells. In this aspect, cell vitality defining the physiological capabilities of the cell is a very important parameter. Measurement of cell vitality is advisable for conducting effective control of the fermentation process at a significant concentration of inhibitory substances that are produced during the process. It is very important for controlling the metabolic activity of yeast cells in fermentation in the brewing industry, in the production of bioethanol, in the bakery industry, etc. The Yeast Vitality Method is based on the determination of the enzyme esterase activity by means of the esterase substrate 5-carboxyfluorescein diacetate (CFDA). In parallel with this analysis, it is important to determine the total number of cells in the yeast suspension. This can be achieved by using the Sofia-Green fluorescent dye, which only stains the dead cells. To determine the total number of cells, it is necessary to treat the cells so that the membrane of the living cells becomes permeable to Sofia-Green. This is achieved by mixing the cell suspension with a lysis reagent for 10 minutes. The combination of these two analyzes results in an effective fermentation control and an increase in the yield and quality of the bio product obtained.


view_comfy Content


CELLCHIP x4 Disposable 25 pcs
Transparent eppendorfs with lysis solution – lyophilized dye SOFIA GREEN Disposable 50 pcs
Colored eppendorfs containing lyophilized 5-carboxyfluoresceindiacetate (CFDA) Disposable 50 pcs
Empty colored eppendorf necessary for mixing the sample and the citarate buffer. Disposable 50 pcs
Automatic pipette tip up to 100µl Disposable 250 pcs
Automatic pipette tip up to 1000µl Disposable 100 pcs
Eppendorfs, containing dry components for buffer preparation – citrate-phosphate buffer pH 4 (Macilvaine buffer) Disposable 5 pcs
15 ml tube Reusable 1 pcs


dns Storage Conditions


The 50 brown-colored eppendorfs with dried 5-carboxyfluorescein diacetate (CFDA) should be stored frozen at ≤ -20 ° C, protected from light.

The remaining components of EASYCOUNTER YC KIT must be stored at -10 ̊C to +40 ̊C, protected from direct sunlight.

Box dimensions (W x D x H): 30x25.5x13.5 cm
Weight < 1.5 kg
Cardboard storage box


save_alt Downloads


INSTRUCTION - BG 2018-09-03 | 712.61KB save_alt

INSTRUCTION - EN 2018-07-31 | 958.39KB save_alt



extension Compatible with


EASYCOUNTER YC YCS EASYCOUNTER YC - Read More

EASYCOUNTER YC Compact YCC EASYCOUNTER YC Compact - Read More


Don't want to miss a thing?
Subscribe to get the latest updates for our products!

MILKOTRONIC LTD
4, Narodni Buditeli Street
8900 Nova Zagora
BULGARIA
tel/fax: + 359 457 670 82
office@autocellcount.com

Copyright © 1997-2018 Autocellcount.com Milkotronic Ltd. All rights reserved. W