Vitality analysis scheme save_alt Download File

The 5-carboxyfluorescein diacetate (CFDA) esterase substrate is used to determine the vitality of yeast. CFDA penetrates the cell membranes of living cells, the diacetate groups are cleaved from non-specific esterases and a fluorescent, charged substrate form (carboxyfluorescein) is obtained that stains the cells in green and flows out of the cells very slowly. In parallel, in a separate sample with yeast cell suspension, the total number of yeast cells was determined using the fluorescent dye Sofia Green. To determine the total number of cells, it is necessary for the membrane of living cells to become permeable to Sofia Green. This is achieved by mixing the cell suspension with a lysis reagent for 10 minutes. Sofia Green penetrates through damaged cell membranes, binds to core nucleus, and cells with damaged membranes turn green.

Other downloads for this product:

User manual 2018-12-17 | 33.46MB save_alt
Инструкция за употреба 2018-12-17 | 31.50MB save_alt
Preparing the sample for determination of Viability 2018-07-06 | 1.13MB save_alt
Initial dilution of yeast suspension 2019-01-03 | 2.29MB save_alt
Viability analysis scheme 2019-01-03 | 983.77KB save_alt
Vitality analysis scheme 2019-01-23 | 2.07MB save_alt
Esquema de análisis de vitalidad. 2019-01-23 | 1.65MB save_alt
Excel table for calculation of Coefficient of variation (CV %) 2018-07-26 | 12.41KB save_alt

4, Narodni Buditeli Street
8900 Nova Zagora
tel/fax: + 359 457 670 82

Copyright © 1997-2020 Milkotronic Ltd. All rights reserved. W