Fluorescent yeast cell counter

EASYCOUNTER YC

PN: YC-S

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EASYCOUNTER YC Compact

METHOD

EASYCOUNTER YC Yeast cell counter is based on fluorescent image cytometry for counting cells. The instrument counts individual yeast cells in suspension by detecting the fluorescence signals of stained DNA in the cell nuclei. It performs viability and vitality analysis. Viability is defined as a percentage of live cells in a whole population. In many cases, toxic effects of chemical or physical factors do not lead directly to cell death but may compromise cell ability to divide without necessarily leading to cell death. The analysis for assessing cell viability provides information only on alive and dead cells in the whole population, but do not provide information about the number of vitality cells. Vitality is defined as the physiological capabilities of cells. It is important to note that cell viability and cell vitality represent two different aspects of cell functions, and both are required for the estimation of the physiological state of a cell after exposure to various types of stressors and chemical or physical factors.

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KEY BENEFITS

> World's lowest price for image cytometer
> Plug & Test
> 20 to 60 sec. analysis time
> High repeatability of the results
> Safe non-mutagenic fluorescence cyanine dye
> Disposable CELLCHIPS
> User Friendly interface
> Rich content report
> Fully-functional 10" Windows tablet

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Cloud Solution

Results are easily stored and accessible at our cloud from any device all over the world or on your own local secure server.

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FIELDS OF ANALYSIS

Our device is used from small breweries, wineries, bakeries and others to large factories and labs, even in educational and state control institutions.

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Worlds lowest cost per test

The high precision viability and vitality yeast cell analysis is affordable for small to large breweries, wineries, bakeries, labs and others.


yeast counting in 4 easy steps
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Demo software

Fully functional demo software with results from real tests of milk samples from animals suffering from subclinical, clinical, yeast and mycoplasma mastitis.

fitness of yeast population
Fitness of yeast population

Our device is suited to be used in all environments and situacions.

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SIMPLE AND ЕASY RECORDKEEPING

Results from the analysis can be printed to the inbuilt printer in short report or exported in pdf and excel in extended report with images and graphs.


Working principle

EASYCOUNTER YC is based on fluorescent microscope technique of counting cells. Thanks to the fluorescent dye, LED optics and CMOS filming Technologies, the analysis of the yeast is precise, reliable and fast.

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Coefficient of variation (CV, %)

EASYCOUNTER YC possesses the largest volume of scanned sample up to 8 µl that guarantee low coefficient of variation, better than the direct microscopic counting.

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Verification as microscope

There is no need of calibration, only a graduated scale on a CELLCHIP is used for verification of the magnification as per requirements of ISO 20391-1:2018 Biotechnology -- Cell counting -- Part 1: General guidance on cell counting methods


Counting area

The counting area of the EASYCOUNTER YC has:
43 times larger area than a standard hemocytometer and
12 times larger area than similar instruments.

counting area of yeast counter

Preparing the sample for yeast cell viability analysis

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Preparing the sample for determination of Viability

Preparing the sample for yeast cell vitality analysis

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Sample preparation for Vitality analysis.

Results from Viability analysis

Analysis time: 40-120 sec
Measuring range: 0 - 10 000 000 cell/ml
Measuring cell size range: 2-30 µm
Colour indication for sub-clinical and clinical mastitis

how it works scc counter

Results from Vitality analysis

Analysis time: 40-120 sec
Measuring range: 0 - 10 000 000 cell/ml
Measuring cell size range: 2-30 µm
Colour indication for sub-clinical and clinical mastitis

how it works scc counter


ballot Features


EASYCOUNTER YC is designed to be used for yeast analysis in the production of beer, bioethanol, bakery, research laboratories. It is not intended for human or animal therapeutic or diagnostic use.

  • High-end direct fluorescent, low magnification microscope with fast autofocus and cell counting software
  • Fast, accurate, reliable yeas count.
  • Possibility of reference analysis
  • Easy readable results within 40 to 120 seconds
  • Takes up to 60 images by computer controlled X:Y movements and then processes these with special image analysis software
  • Stores unlimited number of record in the database
  • Results are color coded
  • Automatic software update and remote service
  • Portable and compact design
  • 10'' fully functional touchscreen tablet
  • Embedded printer
  • Wireless keyboard and mouse
  • Use of 4 channel disposable CELLCHIPS
  • Low power consumption
  • One year full warranty


insert_chart Measuring parameters


Coefficient of variation for Viability

Coefficient of variation (CV, %) of the cell count determined using the fluorescent image cytometer EASYCOUNTER YC and direct observation under microscope at different dilutions of cell suspension with initial concentration 1.3 х 109

Dillution Total cell count Dillution Dead cell count
YC DMCC YC DMCC
50x 7.8% 40% 5x 8.7% 41%
100x 5.6% 38% 10x 4.9% 39%
200x 3.8% 15% 20x 3.7% 18%
400x 2.9% 11% 40x 2.8% 14%
500x 2.5% 8% 50x 2.3% 11%
1000x 3.8% 7.3% 100x 4.8% 9%
* Coefficient of Variation

Coefficient of variation for Vitality

Dillution Total cell count Dillution VItal cell count
YC DMCC YC DMCC
50x 7.8% 40% 5x 8.7% 42%
100x 5.6% 38% 10x 6.1% 41%
200x 3.8% 15% 20x 4.3% 21%
400x 2.9% 11% 40x 3.4% 15%
500x 2.5% 8% 50x 3.1% 11%
1000x 3.8% 7.3% 100x 5.8% 10%
* Coefficient of Variation


cloud_circle Environmental Conditions


Indoor temperature 15-35°С. It is not recommended to place it in a room where the temperature is below 4°С.
Prevent exposing the device to direct sun light.
Prevent exposing to a continuous vibration.
Relative humidity 0-95%.
In a place free of corrosive gazes or other corrosive substances.
In a place free of dust.
10 cm (4 inches) is the minimum required distance for normal air flow.
Do not place any heavy object on the device.


flash_on Electrical Parameters


AC Power Supply voltage: 220V/110V
DC Power Supply voltage: 12V, 5A
Power Consumption: 60W


settings Mechanical Parameters


Dimensions (W x D x H): 390x300x260 mm
Weight lower than 5.5 kg
Plastic cover box


business_center What's in the box?

EASYCOUNTER YC yeast counter1
EASYCOUNTER YC STARTER VIABILITY KIT1
12V power adapter1
Quick start guide1
Automatic pipette up to 100 µL – set at 8 µL1
Automatic pipette up to 1000 µL – set at 1000 µL1
Mini Vortex1
USB wireless keyboard with batteries1
USB wireless mouse with batteries1


contact_support Support

description Настройка на YC Learn More
description Determination the dilution rate Learn More
description Cell viability determination Learn More
description Cell vitality determination Learn More


save_alt Downloads


Excel table for calculation of Coefficient of variation (CV %) 2018-07-26 | 12.41KB save_alt

User manual 2018-09-17 | 31.41MB save_alt

Инструкция за употреба 2018-09-17 | 31.67MB save_alt

Preparing the sample for determination of Viability 2018-07-06 | 1.13MB save_alt

Initial dilution of yeast suspension 2018-06-13 | 1.16MB save_alt

Vitality analysis scheme 2018-06-14 | 1.29MB save_alt

Viability analysis scheme 2018-06-14 | 1.33MB save_alt



layers Consumables


EASYCOUNTER YC VITALITY KIT YC-VT EASYCOUNTER YC VITALITY KIT FOR 50 TESTS - Read More

EASYCOUNTER YC VIABILITY KIT YC-VB EASYCOUNTER YC VIABILITY KIT FOR 200 TESTS - Read More



extension Accessories


EASYCOUNTER EPF - YC TDC EASYCOUNTER EPF - YC 120€ Read More


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MILKOTRONIC LTD
4, Narodni Buditeli Street
8900 Nova Zagora
BULGARIA
tel/fax: + 359 457 670 82
office@autocellcount.com

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