Preparing the sample for determination of Viability save_alt Download File

The EASYCOUNTER YC-1 counts individual cells in suspension by detecting fluorescence signals of stained DNA in the cell nuclei. The staining dye used is SOFIA GREEN. The cell membrane of viable cells is virtually impermeable with regard to SOFIA GREEN, while membrane of non-viable, dead cells is permeable to SOFIA GREEN. The determination of the total concentration of cells requires that the membrane of viable cells is made permeable to SOFIA GREEN. This is achieved by mixing cell suspension with Lysis reagent. After Lysis reagent treatment (10 min), approximately 8 µl of the all cell stained with YO-DEDM 1 and 8 µl of the only dead cells stained with YO-DEDM 1 were loaded into the CELLCHIP. The CELLCHIP is placed in the EASYCOUNTER YC instrument where the cells are automatically enumerated.

Other downloads for this product:

User manual 2018-12-17 | 33.46MB save_alt
Инструкция за употреба 2018-12-17 | 31.50MB save_alt
Preparing the sample for determination of Viability 2018-07-06 | 1.13MB save_alt
Initial dilution of yeast suspension 2019-01-03 | 2.29MB save_alt
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Vitality analysis scheme 2019-01-23 | 2.07MB save_alt
Esquema de análisis de vitalidad. 2019-01-23 | 1.65MB save_alt
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