INSTRUCTION MANUAL save_alt Download File

The EASYCOUNTER YC counts individual yeast cells in suspension by detecting the fluorescence signals of stained DNA in the cell nuclei. The staining dye used is Sofia Green. The cell membrane of viable cells is virtually impermeable with regard to Sofia Green, while non-viable, dead and to a certain extent apoptotic cells, is permeable to Sofia Green. The determination of the total concentration of cells requires the membrane of viable cells to be permeable to Sofia Green. This is achieved by mixing cell suspension with Lysis reagent. After Lysis reagent treatment (10 min), approximately 8 μl of the all cell stained with Sofia Green and 8 μl of the only dead cells stained with Sofia Green are loaded into the CELLCHIP. The CELLCHIP is placed in the EASYCOUNTER YC instrument where the cells are automatically counted.


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Инструкции за употреба 2018-09-03 | 1.65MB save_alt
INSTRUCTION MANUAL 2018-09-03 | 1.60MB save_alt

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