Abstract: A rapid method for SCC with YOYO1 was developed. Dying the cells fluorescent is an advantageous technology with accurate and repeatable results. PMN elastase from bovine milk was obtained and proven by a RP-HPLC. The activity of the PMN elastase was measured and compared with porcine pancreatic elastase. Measuring the PMN elastase activity through created method is helpful for determination of the cattle health.

Keywords: SCC; mastitis; PMN elastase; elastase activity; YOYO1; HPLC; milk;

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