LACTOSCAN SCC Somatic cell counter is based on fluorescent image cytometry for counting cells in milk. It fully comply with the ISO 13366-1 IDF 148-1 Enumeration of somatic cells - Microscopic method (Reference method) with regard to the staining of cells, in the definition of cells to be counted depending on the size and the methodology of the counting. The somatic cell counter offers visual control for excluding accidental errors. The measuring range of the counter is 2 to 30 µm and the results are expressed as number of cells per mL. The results are available within 20 to 60 seconds depending on the type of analysis. There is no need of calibration, except the standard calibration of magnification done by using a graduated scale on a slide as in the microscope.
Unique, 3D, multi-image, patent application protected, sequential scanning process, based on a precise fluorescent optics and low magnification, images analysis software, LACTОSCAN SCC is fast, precise and reliable counter of somatic cells. Via automatic displacement of the mechanism on axles X – Y and liquid lens Z, the device is capturing maximum 60 images. After capturing, the images are being processed by the embedded software and the average result, calculated by using the formula from IDF/ISO 13366, of all the filmed images is displayed. The whole process, after placing the LACTOCHIP in the cartridge, is automatic.
The below described data are based on validation and check of LACTOSCAN SCC done by the “Biotechnologies” department of University “Prof. Asen Zlatarov”, Bulgaria
1. Accuracy of analysis – comparison between LACTOSCAN SCC and Direct microscopic counting of somatic cells (DMSCC)
Images of somatic cells (LACTOSCAN SCC)
Images of somatic cells (DMSCC)
|LACTOSCAN SCC||Standard method|
|NSC (cells/ml)||*CV %||NSC (cells/ml)||*CV %|
|100 000||5 %||100 000||7 %|
|500 000||3 %||400 000||5 %|
|1 000 000||2 %||600 000||4 %|
2. Compatibility – ratio between data from LACTOSCAN SCC and DMSCC
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